Analysis of collagen preservation in bones recovered in archaeological contexts using NIR Hyperspectral Imaging

Damien Vincke, Rebecca Miller, Édith Stassart, Marcel Otte, Pierre Dardenne, Matthew Collins, Keith Wilkinson, John Stewart, Vincent Baeten, Juan Antonio Fernández Pierna

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The scope of this article is to propose an innovative method based on Near Infrared Hyperspectral Chemical Imaging (NIR-HCI) to rapidly and non-destructively evaluate the relative degree of collagen preservation in bones recovered from archaeological contexts. This preliminary study has allowed the evaluation of the potential of the method using bone samples from the Early Upper Palaeolithic, Mesolithic and Neolithic periods at the site of Trou AlWesse in Belgium. NIR-HCI, combined with chemometric tools, has identified specific spectral bands characteristic of collagen. A chemometric model has been built using Partial Least Squares Discriminant Analysis (PLS-DA) to identify bones with and without collagen. This enables the evaluation of the degree of collagen preservation and homogeneity in bones within and between different strata, which has direct implications for archaeological applications (e.g., taphonomic analyses, assemblage integrity) and sample selection for subsequent analyses requiring collagen. Two archaeological applications are presented: comparison between sub-layers in an Early Upper Palaeolithic unit, and evaluation of the range of variability in collagen preservation within a single Holocene stratum. © 2014 Elsevier B.V.
Original languageEnglish
Pages (from-to)181-188
Number of pages8
JournalTalanta
Volume125
DOIs
Publication statusPublished - 1 Jul 2014

Keywords

  • Bone
  • Collagen
  • Near Infrared (NIR) Hyperspectral Imaging
  • Partial Least Squares-Discriminant Analysis (PLS-DA)
  • Principal Component Analysis (PCA)
  • Sorting

Cite this

Vincke, D., Miller, R., Stassart, É., Otte, M., Dardenne, P., Collins, M., ... Fernández Pierna, J. A. (2014). Analysis of collagen preservation in bones recovered in archaeological contexts using NIR Hyperspectral Imaging. Talanta, 125, 181-188. https://doi.org/10.1016/j.talanta.2014.02.044
Vincke, Damien ; Miller, Rebecca ; Stassart, Édith ; Otte, Marcel ; Dardenne, Pierre ; Collins, Matthew ; Wilkinson, Keith ; Stewart, John ; Baeten, Vincent ; Fernández Pierna, Juan Antonio. / Analysis of collagen preservation in bones recovered in archaeological contexts using NIR Hyperspectral Imaging. In: Talanta. 2014 ; Vol. 125. pp. 181-188.
@article{339cdcb7b9834610af8cfa51e452b0ce,
title = "Analysis of collagen preservation in bones recovered in archaeological contexts using NIR Hyperspectral Imaging",
abstract = "The scope of this article is to propose an innovative method based on Near Infrared Hyperspectral Chemical Imaging (NIR-HCI) to rapidly and non-destructively evaluate the relative degree of collagen preservation in bones recovered from archaeological contexts. This preliminary study has allowed the evaluation of the potential of the method using bone samples from the Early Upper Palaeolithic, Mesolithic and Neolithic periods at the site of Trou AlWesse in Belgium. NIR-HCI, combined with chemometric tools, has identified specific spectral bands characteristic of collagen. A chemometric model has been built using Partial Least Squares Discriminant Analysis (PLS-DA) to identify bones with and without collagen. This enables the evaluation of the degree of collagen preservation and homogeneity in bones within and between different strata, which has direct implications for archaeological applications (e.g., taphonomic analyses, assemblage integrity) and sample selection for subsequent analyses requiring collagen. Two archaeological applications are presented: comparison between sub-layers in an Early Upper Palaeolithic unit, and evaluation of the range of variability in collagen preservation within a single Holocene stratum. {\circledC} 2014 Elsevier B.V.",
keywords = "Bone, Collagen, Near Infrared (NIR) Hyperspectral Imaging, Partial Least Squares-Discriminant Analysis (PLS-DA), Principal Component Analysis (PCA), Sorting",
author = "Damien Vincke and Rebecca Miller and {\'E}dith Stassart and Marcel Otte and Pierre Dardenne and Matthew Collins and Keith Wilkinson and John Stewart and Vincent Baeten and {Fern{\'a}ndez Pierna}, {Juan Antonio}",
year = "2014",
month = "7",
day = "1",
doi = "10.1016/j.talanta.2014.02.044",
language = "English",
volume = "125",
pages = "181--188",

}

Vincke, D, Miller, R, Stassart, É, Otte, M, Dardenne, P, Collins, M, Wilkinson, K, Stewart, J, Baeten, V & Fernández Pierna, JA 2014, 'Analysis of collagen preservation in bones recovered in archaeological contexts using NIR Hyperspectral Imaging', Talanta, vol. 125, pp. 181-188. https://doi.org/10.1016/j.talanta.2014.02.044

Analysis of collagen preservation in bones recovered in archaeological contexts using NIR Hyperspectral Imaging. / Vincke, Damien; Miller, Rebecca; Stassart, Édith; Otte, Marcel; Dardenne, Pierre; Collins, Matthew; Wilkinson, Keith; Stewart, John; Baeten, Vincent; Fernández Pierna, Juan Antonio.

In: Talanta, Vol. 125, 01.07.2014, p. 181-188.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Analysis of collagen preservation in bones recovered in archaeological contexts using NIR Hyperspectral Imaging

AU - Vincke, Damien

AU - Miller, Rebecca

AU - Stassart, Édith

AU - Otte, Marcel

AU - Dardenne, Pierre

AU - Collins, Matthew

AU - Wilkinson, Keith

AU - Stewart, John

AU - Baeten, Vincent

AU - Fernández Pierna, Juan Antonio

PY - 2014/7/1

Y1 - 2014/7/1

N2 - The scope of this article is to propose an innovative method based on Near Infrared Hyperspectral Chemical Imaging (NIR-HCI) to rapidly and non-destructively evaluate the relative degree of collagen preservation in bones recovered from archaeological contexts. This preliminary study has allowed the evaluation of the potential of the method using bone samples from the Early Upper Palaeolithic, Mesolithic and Neolithic periods at the site of Trou AlWesse in Belgium. NIR-HCI, combined with chemometric tools, has identified specific spectral bands characteristic of collagen. A chemometric model has been built using Partial Least Squares Discriminant Analysis (PLS-DA) to identify bones with and without collagen. This enables the evaluation of the degree of collagen preservation and homogeneity in bones within and between different strata, which has direct implications for archaeological applications (e.g., taphonomic analyses, assemblage integrity) and sample selection for subsequent analyses requiring collagen. Two archaeological applications are presented: comparison between sub-layers in an Early Upper Palaeolithic unit, and evaluation of the range of variability in collagen preservation within a single Holocene stratum. © 2014 Elsevier B.V.

AB - The scope of this article is to propose an innovative method based on Near Infrared Hyperspectral Chemical Imaging (NIR-HCI) to rapidly and non-destructively evaluate the relative degree of collagen preservation in bones recovered from archaeological contexts. This preliminary study has allowed the evaluation of the potential of the method using bone samples from the Early Upper Palaeolithic, Mesolithic and Neolithic periods at the site of Trou AlWesse in Belgium. NIR-HCI, combined with chemometric tools, has identified specific spectral bands characteristic of collagen. A chemometric model has been built using Partial Least Squares Discriminant Analysis (PLS-DA) to identify bones with and without collagen. This enables the evaluation of the degree of collagen preservation and homogeneity in bones within and between different strata, which has direct implications for archaeological applications (e.g., taphonomic analyses, assemblage integrity) and sample selection for subsequent analyses requiring collagen. Two archaeological applications are presented: comparison between sub-layers in an Early Upper Palaeolithic unit, and evaluation of the range of variability in collagen preservation within a single Holocene stratum. © 2014 Elsevier B.V.

KW - Bone

KW - Collagen

KW - Near Infrared (NIR) Hyperspectral Imaging

KW - Partial Least Squares-Discriminant Analysis (PLS-DA)

KW - Principal Component Analysis (PCA)

KW - Sorting

U2 - 10.1016/j.talanta.2014.02.044

DO - 10.1016/j.talanta.2014.02.044

M3 - Article

VL - 125

SP - 181

EP - 188

ER -

Vincke D, Miller R, Stassart É, Otte M, Dardenne P, Collins M et al. Analysis of collagen preservation in bones recovered in archaeological contexts using NIR Hyperspectral Imaging. Talanta. 2014 Jul 1;125:181-188. https://doi.org/10.1016/j.talanta.2014.02.044