Neandertal and Denisovan DNA from Pleistocene sediments

Viviane Slon, Charlotte Hopfe, Clemens L. Weiß, Fabrizio Mafessoni, Marco De La Rasilla, Carles Lalueza-Fox, Antonio Rosas, Marie Soressi, Monika V. Knul, Rebecca Miller, John R. Stewart, Anatoly P. Derevianko, Zenobia Jacobs, Bo Li, Richard G. Roberts, Michael V. Shunkov, Henry De Lumley, Christian Perrenoud, Ivan Gušić, Željko Kućan & 11 others Pavao Rudan, Ayinuer Aximu-Petri, Elena Essel, Sarah Nagel, Birgit Nickel, Anna Schmidt, Kay Prüfer, Janet Kelso, Hernán A. Burbano, Svante Pääbo, Matthias Meyer

Research output: Contribution to journalArticleResearchpeer-review

45 Citations (Scopus)

Abstract

Although a rich record of Pleistocene human-associated archaeological assemblages exists, the scarcity of hominin fossils often impedes the understanding of which hominins occupied a site. Using targeted enrichment of mitochondrial DNA, we show that cave sediments represent a rich source of ancient mammalian DNA that often includes traces of hominin DNA, even at sites and in layers where no hominin remains have been discovered. By automation-assisted screening of numerous sediment samples, we detected Neandertal DNA in eight archaeological layers from four caves in Eurasia. In Denisova Cave, we retrieved Denisovan DNA in a Middle Pleistocene layer near the bottom of the stratigraphy. Our work opens the possibility of detecting the presence of hominin groups at sites and in areas where no skeletal remains are found.

Original languageEnglish
Pages (from-to)605-608
Number of pages4
JournalScience
Volume356
Issue number6338
DOIs
Publication statusPublished - 12 May 2017
Externally publishedYes

Cite this

Slon, V., Hopfe, C., Weiß, C. L., Mafessoni, F., De La Rasilla, M., Lalueza-Fox, C., ... Meyer, M. (2017). Neandertal and Denisovan DNA from Pleistocene sediments. Science, 356(6338), 605-608. https://doi.org/10.1126/science.aam9695
Slon, Viviane ; Hopfe, Charlotte ; Weiß, Clemens L. ; Mafessoni, Fabrizio ; De La Rasilla, Marco ; Lalueza-Fox, Carles ; Rosas, Antonio ; Soressi, Marie ; Knul, Monika V. ; Miller, Rebecca ; Stewart, John R. ; Derevianko, Anatoly P. ; Jacobs, Zenobia ; Li, Bo ; Roberts, Richard G. ; Shunkov, Michael V. ; De Lumley, Henry ; Perrenoud, Christian ; Gušić, Ivan ; Kućan, Željko ; Rudan, Pavao ; Aximu-Petri, Ayinuer ; Essel, Elena ; Nagel, Sarah ; Nickel, Birgit ; Schmidt, Anna ; Prüfer, Kay ; Kelso, Janet ; Burbano, Hernán A. ; Pääbo, Svante ; Meyer, Matthias. / Neandertal and Denisovan DNA from Pleistocene sediments. In: Science. 2017 ; Vol. 356, No. 6338. pp. 605-608.
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abstract = "Although a rich record of Pleistocene human-associated archaeological assemblages exists, the scarcity of hominin fossils often impedes the understanding of which hominins occupied a site. Using targeted enrichment of mitochondrial DNA, we show that cave sediments represent a rich source of ancient mammalian DNA that often includes traces of hominin DNA, even at sites and in layers where no hominin remains have been discovered. By automation-assisted screening of numerous sediment samples, we detected Neandertal DNA in eight archaeological layers from four caves in Eurasia. In Denisova Cave, we retrieved Denisovan DNA in a Middle Pleistocene layer near the bottom of the stratigraphy. Our work opens the possibility of detecting the presence of hominin groups at sites and in areas where no skeletal remains are found.",
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Slon, V, Hopfe, C, Weiß, CL, Mafessoni, F, De La Rasilla, M, Lalueza-Fox, C, Rosas, A, Soressi, M, Knul, MV, Miller, R, Stewart, JR, Derevianko, AP, Jacobs, Z, Li, B, Roberts, RG, Shunkov, MV, De Lumley, H, Perrenoud, C, Gušić, I, Kućan, Ž, Rudan, P, Aximu-Petri, A, Essel, E, Nagel, S, Nickel, B, Schmidt, A, Prüfer, K, Kelso, J, Burbano, HA, Pääbo, S & Meyer, M 2017, 'Neandertal and Denisovan DNA from Pleistocene sediments', Science, vol. 356, no. 6338, pp. 605-608. https://doi.org/10.1126/science.aam9695

Neandertal and Denisovan DNA from Pleistocene sediments. / Slon, Viviane; Hopfe, Charlotte; Weiß, Clemens L.; Mafessoni, Fabrizio; De La Rasilla, Marco; Lalueza-Fox, Carles; Rosas, Antonio; Soressi, Marie; Knul, Monika V.; Miller, Rebecca; Stewart, John R.; Derevianko, Anatoly P.; Jacobs, Zenobia; Li, Bo; Roberts, Richard G.; Shunkov, Michael V.; De Lumley, Henry; Perrenoud, Christian; Gušić, Ivan; Kućan, Željko; Rudan, Pavao; Aximu-Petri, Ayinuer; Essel, Elena; Nagel, Sarah; Nickel, Birgit; Schmidt, Anna; Prüfer, Kay; Kelso, Janet; Burbano, Hernán A.; Pääbo, Svante; Meyer, Matthias.

In: Science, Vol. 356, No. 6338, 12.05.2017, p. 605-608.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Neandertal and Denisovan DNA from Pleistocene sediments

AU - Slon, Viviane

AU - Hopfe, Charlotte

AU - Weiß, Clemens L.

AU - Mafessoni, Fabrizio

AU - De La Rasilla, Marco

AU - Lalueza-Fox, Carles

AU - Rosas, Antonio

AU - Soressi, Marie

AU - Knul, Monika V.

AU - Miller, Rebecca

AU - Stewart, John R.

AU - Derevianko, Anatoly P.

AU - Jacobs, Zenobia

AU - Li, Bo

AU - Roberts, Richard G.

AU - Shunkov, Michael V.

AU - De Lumley, Henry

AU - Perrenoud, Christian

AU - Gušić, Ivan

AU - Kućan, Željko

AU - Rudan, Pavao

AU - Aximu-Petri, Ayinuer

AU - Essel, Elena

AU - Nagel, Sarah

AU - Nickel, Birgit

AU - Schmidt, Anna

AU - Prüfer, Kay

AU - Kelso, Janet

AU - Burbano, Hernán A.

AU - Pääbo, Svante

AU - Meyer, Matthias

PY - 2017/5/12

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AB - Although a rich record of Pleistocene human-associated archaeological assemblages exists, the scarcity of hominin fossils often impedes the understanding of which hominins occupied a site. Using targeted enrichment of mitochondrial DNA, we show that cave sediments represent a rich source of ancient mammalian DNA that often includes traces of hominin DNA, even at sites and in layers where no hominin remains have been discovered. By automation-assisted screening of numerous sediment samples, we detected Neandertal DNA in eight archaeological layers from four caves in Eurasia. In Denisova Cave, we retrieved Denisovan DNA in a Middle Pleistocene layer near the bottom of the stratigraphy. Our work opens the possibility of detecting the presence of hominin groups at sites and in areas where no skeletal remains are found.

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Slon V, Hopfe C, Weiß CL, Mafessoni F, De La Rasilla M, Lalueza-Fox C et al. Neandertal and Denisovan DNA from Pleistocene sediments. Science. 2017 May 12;356(6338):605-608. https://doi.org/10.1126/science.aam9695